LOCUS       sequence-61262-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINMalE
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    252..273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(288..293,294..311,312..318)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    326..342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     331..353
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     350..366
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     350..366
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             362..535
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(382..399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(382..398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             565..582
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(672..691)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    737..792
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(791..810)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac
      181 gatgatgttt tccgcctcgg ctctcggtac cgtcgacccg actggaaagc gggcagtgag
      241 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      301 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      361 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      541 ccaagctttc tagaccagtt taaacatcac catcaccatc actaagtgat taacctcagg
      601 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
      661 actgagatcg atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
      721 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
      781 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
      841 tatttggttt agagtttggc aacatatgcc catatgtaac tagcataac
//
