LOCUS       sequence-61283-e        5613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, 
            Zhao J, Weng C, Klibanski A
  TITLE     Activation of p53 by MEG3 non-coding RNA.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24731-42. Epub 2007 Jun 13.
  PUBMED    17569660
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24731-42. 
            Epub 2007 Jun 13"
COMMENT     Sequence Label: pCI-MEG3
FEATURES             Location/Qualifiers
     source          1..5613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2727..2848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2738..2757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2792..2811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3029..3484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3116..3135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3326..3347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3551..3570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3670..3692
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3730..3748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3816..3920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3921..3989,3990..4781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4139..4158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4952..5540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5441..5460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcgc agagagggag
     1081 cgcgccttgg ctcgctggcc ttggcggcgg ctcctcagga gagctggggc gcccacgaga
     1141 ggatccctca cccgggtctc tcctcaggga tgacatcatc cgtccacctc cttgtcttca
     1201 aggaccacct cctctccatg ctgagctgct gccaaggggc ctgctgccca tctacacctc
     1261 acgagggcac taggagcacg gtttcctgga tcccaccaac atacaaagca gccactcact
     1321 gacccccagg accaggatgg caaaggatga agaggaccgg aactgaccag ccagctgtcc
     1381 ctcttaccta aagacttaaa ccaatgccct agtgaggggg cattgggcat taagccctga
     1441 cctttgctat gctcatactt tgactctatg agtactttcc tataagtctt tgcttgtgtt
     1501 cacctgctag caaactggag tgtttccctc cccaaggggg tgtcagtctt tgtcgactga
     1561 ctctgtcatc acccttatga tgtcctgaat ggaaggatcc ctttgggaaa ttctcaggag
     1621 ggggacctgg gccaagggct tggccagcat cctgctggca actccaaggc cctgggtggg
     1681 cttctggaat gagcatgcta ctgaatcacc aaaggcacgc ccgacctctc tgaagatctt
     1741 cctatccttt tctgggggaa tggggtcgat gagagcaacc tcctagggtt gttgtgagaa
     1801 ttaaatgaga taaaagaggc ctcaggcagg atctggcata gaggaggtga tcagcaaatg
     1861 tttgttgaaa aggtttgaca ggtcagtccc ttcccacccc tcttgcttgt cttacttgtc
     1921 ttatttattc tccaacagca ctccaggcag cccttgtcca cgggctctcc ttgcatcagc
     1981 caagcttctt gaaaggcctg tctacacttg ctgtcttcct tcctcacctc caatttcctc
     2041 ttcaacccac tgcttcctga ctcgctctac tccgtggaag cacgctcaca aaggcacgtg
     2101 ggccgtggcc cggctgggtc ggctgaagaa ctgcggatgg aagctgcgga agaggccctg
     2161 atggggccca ccatcccgga cccaagtctt cttcctggcg ggcctctcgt ctccttcctg
     2221 gtttgggcgg aagccatcac ctggatgcct acgtgggaag ggacctcgaa tgtgggaccc
     2281 cagcccctct ccagctcgaa atccctccac agccacgggg acaccctgca cctattccca
     2341 cgggacaggc tggacccaga gactctggac ccggggcctc cccttgagta gagacccgcc
     2401 ctctgactga tggacgccgc tgacctgggg tcagacccgt gggctggacc cctgcccacc
     2461 ccgcaggaac cctgaggcct aggggagctg ttgagccttc agtgtctgca tgtgggaagt
     2521 gggctccttc acctacctca cagggctgtt gtgaggggcg ctgtgatgcg gttccaaagc
     2581 acagggcttg gcgcacccca ctgtgctctc aataaatgtg tttcctgtct taacaaaaaa
     2641 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2701 aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2761 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2821 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2881 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2941 aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3001 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     3061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3481 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     3541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5581 gctggccttt tgctcacatg gctcgacaga tct
//
