LOCUS       sequence-61314-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Burr GS, Mitchell CK, Keflemariam YJ, Heidelberger R, O'Brien J
  TITLE     Calcium-dependent binding of calmodulin to neuronal gap junction 
            proteins.
  JOURNAL   Biochem Biophys Res Commun. 2005 Oct 7;335(4):1191-8.
  PUBMED    16112650
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2005-10-7"; volume: "335(4)"; pages: 
            "1191-8"
COMMENT     Sequence Label: PCx34.7IL-pET42
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..49)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(379..390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(421..465)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(481..498)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(508..525)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(573..595)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tagcagccta ggtattaatc aattagtggt ggtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgcctgca ggcgcgccaa ggcctgtaca gaattcggat
      121 ccgttagatc ccttcttgcc ggcgaacctt ggagctcttc ccgtgggtga ggccccgcgg
      181 ggcgttgggg atctccttca cctccaggca gtctggttct tccttccctc ctccgccatc
      241 accaccatgt tgaactaaaa ttccattaat gttgcggagc tttcgagccc cgtcccttcc
      301 cccgtagtcc ctctccatta tgggatagag aaaggagtaa cgccggtctt tctgcttggc
      361 cataatcgcc atggacccgc gtccctcaat accggagcca ccaccggtac ccagatctgg
      421 gctgtccatg tgctggcgtt cgaatttagc agcagcggtt tctttcatac caattgcagt
      481 actaccgcgt ggcaccagac ccgcggagtg atggtgatgg tgatgaccag aaccactagt
      541 tgaaccatcc gattttggag gatggtcgcc accaccaaac gtggcttgcc agccctgcaa
      601 aggccatgct atatacttgc tggatttcaa gtacttatca atttgtggga tagcttcaat
      661 acgtttttta aaacaaacta attttgggaa cgcatccagg cacattgggt ccatgtataa
      721 aacaacatca agagcgtcat acaacatgaa gtcaggatgg gttacatgat caccatttaa
      781 atatgtttta tgacataaac gatcttcgaa cattttcagc atttcaggta gcttgctaag
      841 aaaatcaact t
//
