LOCUS       sequence-61315-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Burr GS, Mitchell CK, Keflemariam YJ, Heidelberger R, O'Brien J
  TITLE     Calcium-dependent binding of calmodulin to neuronal gap junction 
            proteins.
  JOURNAL   Biochem Biophys Res Commun. 2005 Oct 7;335(4):1191-8.
  PUBMED    16112650
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "2005-10-7"; volume: "335(4)"; pages: 
            "1191-8"
COMMENT     Sequence Label: PCx35IL-pET42
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..46)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(341..352)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(383..427)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(443..460)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(470..487)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(535..557)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cagcctaggt attaatcaat tagtggtggt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgcc tgcaggtcga ctctagatgc cctcttgtcg cctcattttg gactttgtag
      121 ttctcatggc cgaattaggg atctctttga cttctaaaca gtctggctcg gcttctttgg
      181 tggagttctc cgtgttctga agtactccat taacaatggt gttctttatc tttttgctct
      241 cgtctcgctt cagtgaatct tgatccttat ccagtgtcag atagactgtt gagtaccgcc
      301 gctccttctg cttggcggat ccccgatatc ccatggaccc gcgtccctca ataccggagc
      361 caccaccggt acccagatct gggctgtcca tgtgctggcg ttcgaattta gcagcagcgg
      421 tttctttcat accaattgca gtactaccgc gtggcaccag acccgcggag tgatggtgat
      481 ggtgatgacc agaaccacta gttgaaccat ccgattttgg aggatggtcg ccaccaccaa
      541 acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat
      601 caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca
      661 ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat
      721 gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca
      781 gcatttcagg tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct
//
