LOCUS       sequence-61320-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter 
            MT, Watnick T, Weimbs T
  TITLE     Polycystin-1 regulates STAT activity by a dual mechanism.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr
            25.
  PUBMED    21518865
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-05-10"; volume: "108(19)"; pages: 
            "7985-90. Epub 2011 Apr 25"
COMMENT     Sequence Label: NTSP-PC1
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             464..493
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             503..532
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             548..565
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(588..605)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    594..818
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac
       61 ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattcg atatggcccg
      121 ctggcgctac cacgccttgc gtggagagct gtaccggccg gcctgggagc cccaggacta
      181 cgagatggtg gagttgttcc tgcgcaggct gcgcctctgg atgggcctca gcaaggtcaa
      241 ggagttccgc cacaaagtcc gctttgaagg gatggagccg ctgccctctc gctcctccag
      301 gggctccaag gtatccccgg atgtgccccc acccagcgct ggctccgatg cctcgcaccc
      361 ctccacctcc tccagccagc tggatgggct gagcgtgagc ctgggccggc tggggacaag
      421 gtgtgagccc gagccctccc gcctccaagc cgtgttcgct cgagaacaaa aactcatctc
      481 agaagaggat ctggggccct tcgaacaaaa actcatctca gaagaggatc tgaatatgca
      541 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
      601 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      661 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      721 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      781 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      841 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      901 ggtggttacg cgca
//
