LOCUS       sequence-61422-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Cho SW, Kim S, Kim JM, Kim JS
  TITLE     Targeted genome engineering in human cells with the Cas9 RNA-guided 
            endonuclease.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507.
  PUBMED    23360966
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507."
COMMENT     Sequence Label: p3s-Cas9HC
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..67)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(41..67)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(68..88)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttatta ggcgtagtcg ggcacgtcgt
       61 aggggtatac ttttctcttt ttctttggag gtccggagcc gccgtcgccg cccagctggc
      121 tcaggtcgat gcgggtctcg tacagaccgg tgatgctctg gtggatcagg gtggcgtcca
      181 gcacctcctt ggtgctggtg tagcgcttgc ggtcgatggt ggtgtcgaag tacttgaagg
      241 cggcgggggc gcccaggttg gtcagggtga acaggtggat gatgttctcg gcctgctcgc
      301 ggatgggctt gtcgcggtgc ttgttgtagg cgctcagcac cttgtccagg ttggcgtcgg
      361 ccaggatcac gcgcttgctg aactcgctga tctgctcgat gatctcgtcc aggtagtgct
      421 tgtgctgctc cacgaacagc tgcttctgct cgttgtcctc ggggctgccc ttcagcttct
      481 cgtagtggct ggccaggtac aggaagttca cgtacttgct gggcagggcc agctcgttgc
      541 ccttctgcag ctcgccggcg ctggccagca tgcgcttgcg gccgttctcc agctcgaaca
      601 ggctgtactt gggcagcttg atgatcaggt ccttcttcac ctccttgtag cccttggcct
      661 ccaggaagtc gatggggttc ttctcgaagc tgctgcgctc catgatggtg atgcccagca
      721 gctccttcac gctcttcagc ttcttgctct tgcccttctc caccttggcc accaccagca
      781 cgctgtaggc cacggtgggg ctgtcgaagc cgccgtactt cttggggtcc cagtccttct
      841 tgcgggcgat cagcttgtcg ctgttgcgct tgggcaggat gctctccttg ctgaagccgc
      901 cggtctgcac ctcggtcttc ttc
//
