LOCUS       sequence-61424-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-RhoA-wt
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..616)
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     primer_bind     complement(665..686)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagtcacaa gacaaggcaa ccagattttt
       61 tcttcccacg tctagcttgc agagcagctc tcgtagccat ttcaaaaacc tctctcactc
      121 catctttggt ctttgctgaa cactccatgt acccaaaagc gccaatcctg tttgccatat
      181 ctctgccttc ttcaggtttc accggctcct gcttcatctt ggctagctcc cgccttgtgt
      241 gctcatcatt ccgaagatcc ttcttattcc caaccaggat gatgggcacg ttgggacaga
      301 aatgcttgac ttctggggtc cacttttctg ggatgttttc taaactatca gggctgtcga
      361 tggaaaaaca catcagtata acatcggtat ctgggtagga gaggggcctc aggcgatcat
      421 aatcttcctg cccagctgtg tcccacaaag ccaactctac ctgctttcca tccacctcga
      481 tatctgccac atagttctca aacactgtgg gcacatacac ctctgggaac tggtccttgc
      541 tgaagactat gagcaagcat gtctttccac aggctccatc accaacaatc accagtttct
      601 tccggatggc agccatgaat tccttgtaca gctcgtccat gccgagagtg atcccggcgg
      661 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      721 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      781 tgttctgctg gtagtggtcg gcgagctgca cg
//
