LOCUS       sequence-61426-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TRIF-CFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(24..737,738..740,741..743))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     418..437
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actatagaat agggccctct agattacttg tacagctcgt ccatgccgag agtgatcccg
       61 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      121 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      181 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      241 atcttgaagt gggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg
      301 ctgatgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      361 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      421 ttgtagttgc cgtcgtcctt gaagaagatg gtacgctcct ggacgtagcc ttcgggcatg
      481 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      541 ccccaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      601 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      661 aacctgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      721 agctcctcgc ccttgctcac catctcgagt tctgcctcct gcgtcttgtc ctcgggcgcc
      781 tgggaccctc tctggttcca catgtggttg ttcagcccca gctgtaccat ctgtgcgtgg
      841 tggataatga ggggttgcag ccctgggctc tga
//
