LOCUS       sequence-61435-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC
  TITLE     Pyruvate kinase M2 is a phosphotyrosine-binding protein.
  JOURNAL   Nature. 2008 Mar 13;452(7184):181-6. doi: 10.1038/nature06667.
  PUBMED    18337815
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2008-03-13- 13"; volume: "452"; issue: "7184"; 
            pages: "181-6"
COMMENT     Sequence Label: pET-28a-hPKM1
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             105..137
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agcatgactg
      121 gtggacagca aatgggtcgc ggatccgaat tcatgtcgaa gccccatagt gaagccggga
      181 ctgccttcat tcagacccag cagctgcacg cagccatggc tgacacattc ctggagcaca
      241 tgtgccgcct ggacattgat tcaccaccca tcacagcccg gaacactggc atcatctgta
      301 ccattggccc agcttcccga tcagtggaga cgttgaagga gatgattaag tctggaatga
      361 atgtggctcg tctgaacttc tctcatggaa ctcatgagta ccatgcggag accatcaaga
      421 atgtgcgcac agccacggaa agctttgctt ctgaccccat cctctaccgg cccgttgctg
      481 tggctctaga cactaaagga cctgagatcc gaactgggct catcaagggc agcggcactg
      541 cagaggtgga gctgaagaag ggagccactc tcaaaatcac gctggataac gcctacatgg
      601 aaaagtgtga cgagaacatc ctgtggctgg actacaagaa catctgcaag gtggtggaag
      661 tgggcagcaa gatctacgtg gatgatgggc ttatttctct ccaggtgaag cagaaaggtg
      721 ccgacttcct ggtgacggag gtggaaaatg gtggctcctt gggcagcaag aagggtgtga
      781 accttcctgg ggctgctgtg gacttgcctg ctgtgtcgga gaaggacatc caggatctga
      841 agtttggggt cgagcaggat gttgatatgg tgtttgcgtc attcatccgc aaggcatctg
      901 atgtccatga agttaggaag gtcctgggag agaagggaa
//
