LOCUS       sequence-61438-e         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 
            2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson 
            DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC
  TITLE     In vivo genome editing using a high-efficiency TALEN system.
  JOURNAL   Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 
            2012 Sep 23.
  PUBMED    23000899
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11537"; journalName: "Nature"; date: "2012-11-1- 1"; 
            volume: "491"; issue: "7422"; pages: "114-8"
COMMENT     Sequence Label: pRCIscript-crhr1_TAL1A
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             96..122
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 tttggcagat ctaactcgag aaagatattg tatatatcgt aacaatagga ggttcaacaa
       61 tggcttcctc ccctccaaag aaaaagagaa aggttagttg gaaggacgca agtggttggt
      121 ctagagtgga tctacgcacg ctcggctaca gtcagcagca gcaagagaag atcaaaccga
      181 aggtgcgttc gacagtggcg cagcaccacg aggcactggt gggccatggg tttacacacg
      241 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcacgtatc
      301 agcacataat cacggcgttg ccagaggcga cacacgaaga catcgttggc gtcggcaaac
      361 agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga tgcgggggag ttgagaggtc
      421 cgccgttaca gttggacaca ggccaacttg tgaagattgc aaaacgtggc ggcgtgaccg
      481 caatggaggc agtgcatgca tcgcgcaatg cactgacggg tgcccccctg aacctgaccc
      541 cggaccaagt ggtggctatc gccagcaaca aggg
//
