LOCUS       sequence-61454-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-ATTA
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(594..612)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(595..612)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(630..646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(630..646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(643..665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    654..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(678..684,685..702,703..708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    723..744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgattgggc cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgcccttg ccgtcctagc gcgtctcggc tcaccccaga gcaggtcgtg gcaatcgcct
      121 ccaacattgg cgggaaacag gcactcgaga ctgtccagcg cctgcttccc gtgctgtgcc
      181 aagcgcacgg actcacccca gagcaggtcg tggccattgc ctcgaatgga gggggcaaac
      241 aggcgttgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac ggcctcaccc
      301 cagagcaggt cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac
      361 aacgattgct gccggtgctg tgccaagcgc acggattaac cccagagcag gtcgtggcaa
      421 tcgcctccaa cattggcggg aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc
      481 tgtgccaagc gcacggactc agagacgtgg gcacaagggc gaattccagc acactggcgg
      541 ccgttactag tggatccgag ctcggtacca agcttgatgc atagcttgag tattctatag
      601 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt c
//
