LOCUS       sequence-61491-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-TTAT
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(612..628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(612..628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(625..647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    636..652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(660..666,667..684,685..690))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    705..726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(843..860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggcgaattg ggccctctag atgcatgctt cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttagtatct ttcctgtgcc cacgtctctg agcccgtgcg cttggcacag
      121 caccggcagc aatcgttgta cggtttccaa cgcctgtttg ccccctccat tcgaggcaat
      181 ggccacgacc tgctctgggg ttaatccgtg cgcttggcac agcacgggaa gcaggcgctg
      241 gacagtctcg agtgcctgtt tcccgccaat gttggaggcg attgccacga cctgctctgg
      301 ggtgaggccg tgcgcttggc acagcaccgg cagcaatcgt tgtacggttt ccaacgcctg
      361 tttgccccct ccattcgagg caatggccac gacctgctct ggggtgagtc cgtgcgcttg
      421 gcacagcacc ggcagcaatc gttgtacggt ttccaacgcc tgtttgcccc ctccattcga
      481 ggcaatggcc acgacctgct ctggggttaa gcgagacgcg ctaggacggc atctatataa
      541 gggcgaattc cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg
      601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      901 tcaaaggcgg taatacggtt atccacagat cagggatacg cagaaaacat gtgacaaagg
      961 cagcaaaggc agac
//
