LOCUS       sequence-61529-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-ACAA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(603..621)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(604..621)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(639..655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(639..655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(652..674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    663..679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(687..693,694..711,712..717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    732..753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgaattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 aattcgccct tctttcctgt gcccacgtct ctgagtccgt gcgcttggca cagcacggga
      121 agcaggcgct ggacagtctc gagtgcctgt ttcccgccaa tgttggaggc gattgccacg
      181 acctgctctg gggttaatcc gtgcgcttgg cacagcacgg gaagcaggcg ctggacagtc
      241 tcgagtgcct gtttcccgcc aatgttggag gcgattgcca cgacctgctc tggggtgagg
      301 ccgtgcgctt ggcacagcac aggcaacagc ctctgtactg tttccaaggc ttgctttcct
      361 ccgtcgtggc ttgcgatcgc cacgacctgc tctggggtta atccgtgcgc ttggcacagc
      421 acgggaagca ggcgctggac agtctcgagt gcctgtttcc cgccaatgtt ggaggcgatt
      481 gccacgacct gctctggggt gagccgagac gcgctaggac ggcaagggcg aattccagca
      541 cactggcggc cgttactagt ggatccgagc tcggtaccaa gcttgatgca tagcttgagt
      601 attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga
      661 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      721 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      841 ggtttgcgta ttgggcgctc ttccgcttcc tcg
//
