LOCUS       sequence-61534-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-AACA
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(597..615)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(598..615)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(633..649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(633..649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(646..668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    657..673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(681..687,688..705,706..711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    726..747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(864..881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg
       61 cagaattcgc ccttgccgtc ctagcgcgtc tcggctcacc ccagagcagg tcgtggcaat
      121 cgcctccaac attggcggga aacaggcact cgagactgtc cagcgcctgc ttcccgtgct
      181 gtgccaagcg cacggactca ccccagagca ggtcgtggca atcgcctcca acattggcgg
      241 gaaacaggca ctcgagactg tccagcgcct gcttcccgtg ctgtgccaag cgcacggcct
      301 caccccagag caggtcgtgg cgatcgcaag ccacgacgga ggaaagcaag ccttggaaac
      361 agtacagagg ctgttgcctg tgctgtgcca agcgcacgga ttaaccccag agcaggtcgt
      421 ggcaatcgcc tccaacattg gcgggaaaca ggcactcgag actgtccagc gcctgcttcc
      481 cgtgctgtgc caagcgcacg gactcagaga cgtgggcaag ggcgaattcc agcacactgg
      541 cggccgttac tagtggatcc gagctcggta ccaagcttga tgcatagctt gagtattcta
      601 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      781 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      841 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcgg
//
