LOCUS       sequence-61540-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-AAGG
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(594..612)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(595..612)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(630..646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(630..646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(643..665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    654..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(678..684,685..702,703..708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    723..744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(862..879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt gccgtcctag cgcgtctcgg ctcaccccag agcaggtcgt ggcaatcgcc
      121 tccaacattg gcgggaaaca ggcactcgag actgtccagc gcctgcttcc cgtgctgtgc
      181 caagcgcacg gactcacccc agagcaggtc gtggcaatcg cctccaacat tggcgggaaa
      241 caggcactcg agactgtcca gcgcctgctt cccgtgctgt gccaagcgca cggcctcacc
      301 ccagagcagg tcgtggcaat cgcgagcaat aacggcggaa aacaggcttt ggaaacggtg
      361 cagaggctcc ttccagtgct gtgccaagcg cacggattaa ccccagagca ggtcgtggca
      421 atcgcgagca ataacggcgg aaaacaggct ttggaaacgg tgcagaggct ccttccagtg
      481 ctgtgccaag cgcacggact cagagacgtg ggcaaagggc gaattccagc acactggcgg
      541 ccgttactag tggatccgag ctcggtacca agcttgatgc atagcttgag tattctatag
      601 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      841 attgggcgct cttccgcttt cctcgctcac tgactcgctg c
//
