LOCUS       sequence-61545-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CACT
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(615..633)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(616..633)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(651..667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(651..667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(664..686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    675..691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(699..705,706..723,724..729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    744..765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(882..899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg
       61 cagaattcgc ccttgatgcc gtcctagcgc gtctcggctc accccagagc aggtcgtggc
      121 gatcgcaagc cacgacggag gaaagcaagc cttggaaaca gtacagaggc tgttgcctgt
      181 gctgtgccaa gcgcacggac tcaccccaga gcaggtcgtg gcaatcgcct ccaacattgg
      241 cgggaaacag gcactcgaga ctgtccagcg cctgcttccc gtgctgtgcc aagcgcacgg
      301 cctcacccca gagcaggtcg tggcgatcgc aagccacgac ggaggaaagc aagccttgga
      361 aacagtacag aggctgttgc ctgtgctgtg ccaagcgcac ggattaaccc cagagcaggt
      421 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct
      481 gccggtgctg tgccaagcgc acggactcag agacgtgggc acaggaaaga tacttaaggg
      541 cgaattccag cacactggcg gccgttacta gtggatccga gctcggtacc aagcttgatg
      601 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      661 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      721 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      781 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      841 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggttc
      961 caacgg
//
