LOCUS       sequence-61559-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GCGA
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(614..632)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(615..632)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(650..666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(650..666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(663..685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    674..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(698..704,705..722,723..728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    743..764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(881..898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttgatgccg tcctagcgcg tctcggctca ccccagagca ggtcgtggca
      121 atcgcgagca ataacggcgg aaaacaggct ttggaaacgg tgcagaggct ccttccagtg
      181 ctgtgccaag cgcacggact gaccccagag caggtcgtgg cgatcgcaag ccacgacgga
      241 ggaaagcaag ccttggaaac agtacagagg ctgttgcctg tgctgtgcca agcgcacggc
      301 ctcaccccag agcaggtcgt ggcaatcgcg agcaataacg gcggaaaaca ggctttggaa
      361 acggtgcaga ggctccttcc agtgctgtgc caagcgcacg gattaacccc agagcaggtc
      421 gtggcaatcg cctccaacat tggcgggaaa caggcactcg agactgtcca gcgcctgctt
      481 cccgtgctgt gccaagcgca cggactcaga gacgtgggca caggaaagat acttaagggc
      541 gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca agcttgatgc
      601 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
      661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      781 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      841 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      901 gctcgtcgtc gt
//
