LOCUS       sequence-61561-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GCCT
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(612..628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(612..628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(625..647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    636..652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(660..666,667..684,685..690))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    705..726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(843..860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttatataga ttccgtccta gcgcgtctcg gctcacccca gagcaggtcg
      121 tggcaatcgc gagcaataac ggcggaaaac aggctttgga aacggtgcag aggctccttc
      181 cagtgctgtg ccaagcgcac ggactgaccc cagagcaggt cgtggcgatc gcaagccacg
      241 acggaggaaa gcaagccttg gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc
      301 acggcctcac cccagagcag gtcgtggcga tcgcaagcca cgacggagga aagcaagcct
      361 tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc gcacggatta accccagagc
      421 aggtcgtggc cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat
      481 tgctgccggt gctgtgccaa gcgcacggac tcagagacgt gggcacagga aagatactaa
      541 gggcgaattc cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg
      601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      841 tcctcgctca ctgactcgct gcgctcggtc gttcggt
//
