LOCUS       sequence-61578-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CGGC
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(593..611)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(594..611)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(629..645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(642..664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    653..669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(677..683,684..701,702..707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    722..743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgaattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 aattcgccct tctttcctgt gcccacgtct ctgagtccgt gcgcttggca cagcacaggc
      121 aacagcctct gtactgtttc caaggcttgc tttcctccgt cgtggcttgc gatcgccacg
      181 acctgctctg gggttaatcc gtgcgcttgg cacagcactg gaaggagcct ctgcaccgtt
      241 tccaaagcct gttttccgcc gttattgctc gcgattgcca cgacctgctc tggggtgagg
      301 ccgtgcgctt ggcacagcac tggaaggagc ctctgcaccg tttccaaagc ctgttttccg
      361 ccgttattgc tcgcgattgc cacgacctgc tctggggtga gtccgtgcgc ttggcacagc
      421 acaggcaaca gcctctgtac tgtttccaag gcttgctttc ctccgtcgtg gcttgcgatc
      481 gccacgacct gctctggggt gagccgagac gcgaagggcg aattccagca cactggcggc
      541 cgttactagt ggatccgagc tcggtaccaa gcttgatgca tagcttgagt attctatagt
      601 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      841 ttgggcgctc ttccgcttcc tcgctcactg ac
//
