LOCUS       sequence-61599-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GATG
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(534..552)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(535..552)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(570..586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(583..605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(618..624,625..642,643..648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    663..684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(801..818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atntngcgcg tctcggctca ccccagagca ggtcgtggca atcgcgagca ataacggcgg
       61 aaaacaggct ttggaaacgg tgcagaggct ccttccagtg ctgtgccaag cgcacggact
      121 gaccccagag caggtcgtgg caatcgcctc caacattggc gggaaacagg cactcgagac
      181 tgtccagcgc ctgcttcccg tgctgtgcca agcgcacggc ctcaccccag agcaggtcgt
      241 ggccattgcc tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc
      301 ggtgctgtgc caagcgcacg gattaacccc agagcaggtc gtggcaatcg cgagcaataa
      361 cggcggaaaa caggctttgg aaacggtgca gaggctcctt ccagtgctgt gccaagcgca
      421 cggactcaga gacgtgggca caggaaagat acttaagggc gaattccagc acactggcgg
      481 ccgttactag tggatccgag ctcggtacca agcttgatgc atagcttgag tattctatag
      541 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      601 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      661 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      721 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      781 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt cgt
//
