LOCUS       sequence-61606-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-CCTT
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(614..632)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(615..632)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(650..666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(650..666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(663..685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    674..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(698..704,705..722,723..728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    743..764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgaattgggc cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgccctta gtatctttcc tgtgcccacg tctctgagtc cgtgcgcttg gcacagcacc
      121 ggcagcaatc gttgtacggt ttccaacgcc tgtttgcccc ctccattcga ggcaatggcc
      181 acgacctgct ctggggttaa tccgtgcgct tggcacagca ctggcagcaa tcgttgtacg
      241 gtttccaacg cctgtttgcc ccctccattc gaggcaatgg ccacgacctg ctctggggtg
      301 aggccgtgcg cttggcacag cacaggcaac agcctctgta ctgtttccaa ggcttgcttt
      361 cctccgtcgt ggcttgcgat cgccacgacc tgctctgggg tgagtccgtg cgcttggcac
      421 agcacaggca acagcctctg tactgtttcc aaggcttgct ttcctccgtc gtggcttgcg
      481 atcgccacga cctgctctgg ggtgagccga gacgcgctag gacggcatct atataagggc
      541 gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca agcttgatgc
      601 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
      661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      781 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      841 cggggagagg cggtttgcgt attgggcgct
//
