LOCUS       sequence-61614-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GAGC
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(613..629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(613..629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(626..648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    637..653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(661..667,668..685,686..691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    706..727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(844..861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttaagtatc tttcctgtgc ccacgtctct gagtccgtgc gcttggcaca
      121 gcacaggcaa cagcctctgt actgtttcca aggcttgctt tcctccgtcg tggcttgcga
      181 tcgccacgac ctgctctggg gttaatccgt gcgcttggca cagcactgga aggagcctct
      241 gcaccgtttc caaagcctgt tttccgccgt tattgctcgc gattgccacg acctgctctg
      301 gggtgaggcc gtgcgcttgg cacagcacgg gaagcaggcg ctggacagtc tcgagtgcct
      361 gtttcccgcc aatgttggag gcgattgcca cgacctgctc tggggtgagt ccgtgcgctt
      421 ggcacagcac tggaaggagc ctctgcaccg tttccaaagc ctgttttccg ccgttattgc
      481 tcgcgattgc cacgacctgc tctggggtga gccgagacgc gctaggacgg catctatata
      541 agggcgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
      601 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      661 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      721 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      901 ctcaaaggcg gtaatacgta caaaacagga acaga
//
