LOCUS       sequence-61627-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GACC
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(604..622)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(605..622)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(640..656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(640..656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(653..675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    664..680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(688..694,695..712,713..718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    733..754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(871..888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctctg atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttgccgtcct agcgcgtctc ggctcacccc agagcaggtc gtggcaatcg
      121 cgagcaataa cggcggaaaa caggctttgg aaacggtgca gaggctcctt ccagtgctgt
      181 gccaagcgca cggactcacc ccagagcagg tcgtggcaat cgcctccaac attggcggga
      241 aacaggcact cgagactgtc cagcgcctgc ttcccgtgct gtgccaagcg cacggcctca
      301 ccccagagca ggtcgtggcg atcgcaagcc acgacggagg aaagcaagcc ttggaaacag
      361 tacagaggct gttgcctgtg ctgtgccaag cgcacggatt aaccccagag caggtcgtgg
      421 cgatcgcaag ccacgacgga ggaaagcaag ccttggaaac agtacagagg ctgttgcctg
      481 tgctgtgcca agcgcacgga ctcagagacg tgggcacagg aaagaagggc gaattccagc
      541 acactggcgg ccgttactag tggatccgag ctcggtacca agcttgatgc atagcttgag
      601 tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat cacaatcngn
      961 naccagaaan tnacaagcac aagcagacta agcntttg
//
