LOCUS       sequence-61631-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GACA
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(602..620)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(603..620)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(638..654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(651..673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    662..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(686..692,693..710,711..716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    731..752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(869..886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgaattgg gccctctgat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt gccgtcctag cgcgtctcgg ctcaccccag agcaggtcgt ggcaatcgcg
      121 agcaataacg gcggaaaaca ggctttggaa acggtgcaga ggctccttcc agtgctgtgc
      181 caagcgcacg gactcacccc agagcaggtc gtggcaatcg cctccaacat tggcgggaaa
      241 caggcactcg agactgtcca gcgcctgctt cccgtgctgt gccaagcgca cggcctcacc
      301 ccagagcagg tcgtggcgat cgcaagccac gacggaggaa agcaagcctt ggaaacagta
      361 cagaggctgt tgcctgtgct gtgccaagcg cacggattaa ccccagagca ggtcgtggca
      421 atcgcctcca acattggcgg gaaacaggca ctcgagactg tccagcgcct gcttcccgtg
      481 ctgtgccaag cgcacggact cagagacgtg ggcacaggaa agaagggcga attccagcac
      541 actggcggcc gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta
      601 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa gcgtatagtt ccaanagnac gaanagaga
//
