LOCUS       sequence-61637-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINK
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..712
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     670..692
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    795..816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(831..836,837..854,855..861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    869..885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatggca catcaccatc accatcacat
       61 gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt
      121 ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg
      181 gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg
      241 tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat
      301 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
      361 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
      421 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
      481 atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
      541 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
      601 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
      661 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct
      721 gagcagcggt ctggaagttc tgtttcaggg taccgtcgac ccgactggaa agcgggcagt
      781 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      841 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaattt
//
