LOCUS       sequence-61658-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   O'Neill PR, Karunarathne WK, Kalyanaraman V, Silvius JR, Gautam N
  TITLE     G-protein signaling leverages subunit-dependent membrane affinity to
            differentially control betagamma translocation to intracellular 
            membranes.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 
            10.1073/pnas.1205345109. Epub 2012 Dec 3.
  PUBMED    23213235
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1205345109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-18- 18"; volume: "109"; issue: "51"; pages: 
            "E3568-77"
COMMENT     Sequence Label: Venus-G protein Beta2 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             132..383
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     320..341
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccaccat ggacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
      181 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      241 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaaactg agcaaagacc
      301 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      361 tcggcatgga cgagctgtac aagggcggat ccgggggagg gagtgagctg gagcaactga
      421 gacaggaggc cgagcagctc cggaaccaga tccgggatgc ccgaaaagca tgtggggact
      481 caacactgac ccagatcaca gctgggctgg acccagtggg gagaatccag atgaggaccc
      541 ggaggaccct ccgtgggcac ctggcaaaga tctatgccat gcactggggg accgactcaa
      601 ggctgctggt cagcgcctcc caggatggga agctcatcat ctgggacagc tacaccacca
      661 acaaggtcca cgccatcccg ctgcgctcct cctgggtaat gacctgtgcc tacgcgccct
      721 cagggaactt tgtggcctgt gggggggttg gacaacatct gctccatcta cagcctcaag
      781 acccgcgagg gcaacgtcag ggtcagccgg gagctgcctg gccacactgg gtacctgtcg
      841 tgttgccgct tcctggatga caaccaaatc atcaccagct ctggggatac cacctgtgcc
      901 ctg
//
