LOCUS       sequence-61701-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_20aa
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     218..239
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(327..551)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     promoter        complement(611..814)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(640..660)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
       61 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
      121 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      181 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      241 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga
      301 tcctgtcgac caagcttccc caccacacct ctgagacgga gtaccaggtg cagggtggac
      361 tctttctgga tgttgtagtc agacagggtg cgcccatctt ccagctgttt tccagcaaag
      421 atcagcctct gctggtcagg agggatgcct tccttatctt ggatctttgc cttgacattc
      481 tcaatggtgt cactgggctc aacctcgagg gtgatggtct taccagtcag agtcttcacg
      541 aagatctgca tggatccgag ctcgagatct gagtccggta gcgctagcgg atctgacggt
      601 tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      661 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
      721 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
      781 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
      841 agatgtactg ccaagtagga aagtcc
//
