LOCUS       sequence-61702-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_15aa
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      280..289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(326..550)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     promoter        complement(610..813)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(639..659)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
       61 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
      121 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      181 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      241 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
      301 cctgtcgacc aagcttcccc accacacctc tgagacggag taccaggtgc agggtggact
      361 ctttctggat gttgtagtca gacagggtgc gcccatcttc cagctgtttt ccagcaaaga
      421 tcagcctctg ctggtcagga gggatgcctt ccttatcttg gatctttgcc ttgacattct
      481 caatggtgtc actgggctca acctcgaggg tgatggtctt accagtcaga gtcttcacga
      541 agatctgcat ggatccgagc tcgagatctg agtccggtag cgctagcgga tctgacggtt
      601 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      661 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
      721 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
      781 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
      841 gatgtactgc caag
//
