LOCUS       sequence-61707-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GGAA
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(599..617)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(600..617)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    659..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(683..689,690..707,708..713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(866..883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgattgggcc ctctgatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
       61 cgcccttgcc gtcctagcgc gtctcggctc accccagagc aggttgtggc aatcgcgagc
      121 aataacggcg gaaaacaggc tttggaaacg gtgcagaggc tccttccagt gctgtgccaa
      181 gcgcacggcc tcaccccaga gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag
      241 gctttggaaa cggtgcagag gctccttcca gtgctgtgcc aagcgcacgg cctcacccca
      301 gagcaggtcg tggcaatcgc ctccaacatt ggcgggaaac aggcactcga gactgtccag
      361 cgcctgcttc ccgtgctgtg ccaagcgcac ggattaaccc cagagcaggt cgtggcaatc
      421 gcctccaaca ttggcgggaa acaggcactc gagactgtcc agcgcctgct tcccgtgctg
      481 tgccaagcgc acggactcag agacgtgggc acaggaaaga agggcgaatt ccagcacact
      541 ggcggccgtt actagtggat ccgagctcgg taccaagctt gatgcatagc ttgagtattc
      601 tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatc
//
