LOCUS       sequence-61714-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GARTA
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             193..213
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tatacatatg caccatcatc atcatcattc ttctggtgta
      181 gatctgggta ccgagaacct gtacttccaa tccatggcag cccgagtact tataattggc
      241 agtggaggaa gggaacatac gctggcctgg aaacttgcac agtctcatca tgtcaaacaa
      301 gtgttggttg ccccaggaaa cgcaggcact gcctgctctg aaaagatttc aaataccgcc
      361 atctcaatca gtgaccacac tgcccttgct caattctgca aagagaagaa aattgaattt
      421 gtagttgttg gaccagaagc acctctggct gctgggattg ttgggaacct gaggtctgca
      481 ggagtgcaat gctttggccc aacagcagaa gcggctcagt tagagtccag caaaaggttt
      541 gccaaagagt ttatggacag acatggaatc ccaaccgcac aatggaaggc tttcaccaaa
      601 cctgaagaag cctgcagctt cattttgagt gcagacttcc ctgctttggt tgtgaaggcc
      661 agtggtcttg cagctggaaa aggggtgatt gttgcaaaga gcaaagaaga ggcctgcaaa
      721 gctgtacaag agatcatgca ggagaaagcc tttggggcag ctggagaaac aattgtcatt
      781 gaagaacttc ttgacggaga a
//
