LOCUS       sequence-61715-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GGCT
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(592..610)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(593..610)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(628..644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(641..663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    652..668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(676..682,683..700,701..706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    721..742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(859..876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt ctttcctgtg cccacgtctc tgagtccgtg cgcttggcac agcaccggca
      121 gcaatcgttg tacggtttcc aacgcctgtt tgccccctcc attcgaggca atggccacga
      181 cctgctctgg ggttaatccg tgcgcttggc acagcacagg caacagcctc tgtactgttt
      241 ccaaggcttg ctttcctccg tcgtggcttg cgatcgccac gacctgctct ggggtgaggc
      301 cgtgcgcttg gcacagcact ggaaggagcc tctgcaccgt ttccaaagcc tgttttccgc
      361 cgttattgct cgcgattgcc acgacctgct ctggggtgag tccgtgcgct tggcacagca
      421 ctggaaggag cctctgcacc gtttccaaag cctgttttcc gccgttattg ctcgcgattg
      481 ccacgacctg ctctggggtg agccgagacg cgaagggcga attccagcac actggcggcc
      541 gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta ttctatagtg
      601 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc t
//
