LOCUS       sequence-61721-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TTTC
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(611..627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(624..646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    635..651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(659..665,666..683,684..689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    704..725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(842..859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctctg atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttatatagat tccgtcctag cgcgtctcgg ctcaccccag agcaggtcgt
      121 ggccattgcc tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc
      181 ggtgctgtgc caagcgcacg gactgacccc agagcaggtc gtggccattg cctcgaatgg
      241 agggggcaaa caggcgttgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca
      301 cggcctcacc ccagagcagg tcgtggccat tgcctcgaat ggagggggca aacaggcgtt
      361 ggaaaccgta caacgattgc tgccggtgct gtgccaagcg cacggattaa ccccagagca
      421 ggtcgtggcg atcgcaagcc acgacggagg aaagcaagcc ttggaaacag tacagaggct
      481 gttgcctgtg ctgtgccaag cgcacggact cagagacgtg ggcacaggaa agatactaag
      541 ggcgaattcc agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttgg
      601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      901 caaaggcggt aatacggtta tccacataat caggggatan cgcaggaaag aacatgtgag
      961 caaaggcaca aagcagacta aagcctgtgg ttctgtcgct
//
