LOCUS       sequence-61722-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GGTA
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(637..653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(650..672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    661..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(685..691,692..709,710..715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    730..751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgattggg ccctctgatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgcccttg ccgtcctagc gcgtctcggc tcaccccaga gcaggtcgtg gcaatcgcga
      121 gcaataacgg cggaaaacag gctttggaaa cggtgcagag gctccttcca gtgctgtgcc
      181 aagcgcacgg actcacccca gagcaggtcg tggcaatcgc gagcaataac ggcggaaaac
      241 aggctttgga aacggtgcag aggctccttc cagtgctgtg ccaagcgcac ggcctcaccc
      301 cagagcaggt cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac
      361 aacgattgct gccggtgctg tgccaagcgc acggattaac cccagagcag gtcgtggcaa
      421 tcgcctccaa cattggcggg aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc
      481 tgtgccaagc gcacggactc agagacgtgg gcacaggaaa gaagggcgaa ttccagcaca
      541 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttgatgcata gcttgagtat
      601 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcg
//
