LOCUS       sequence-61745-e        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TTCA
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(611..629)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(612..629)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(647..663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(660..682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(695..701,702..719,720..725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    740..761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(878..895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgattggg ccctctagan ncatgctcga gcggccgcca gtgtgatgga tatctgcaga
       61 attcgccctt gatgccgtcc tagcgcgtct cggctcaccc cagagcaggt cgtggccatt
      121 gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct gccggtgctg
      181 tgccaagcgc acggactcac cccagagcag gtcgtggcca ttgcctcgaa tggagggggc
      241 aaacaggcgt tggaaaccgt acaacgattg ctgccggtgc tgtgccaagc gcacggcctc
      301 accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc cttggaaaca
      361 gtacagaggc tgttgcctgt gctgtgccaa gcgcacggat taaccccaga gcaggtcgtg
      421 gcaatcgcct ccaacattgg cgggaaacag gcactcgaga ctgtccagcg cctgcttccc
      481 gtgctgtgcc aagcgcacgg actcagagac gtgggcacag gaaagatact taagggcgaa
      541 ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttgatgcata
      601 gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc
      661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      721 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      781 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtatnn nnncnnnnnn
      961 gnannnnana nngncaagnn nnnnanaaaa atttgnnaaa naanannngc ccannaanan
     1021 gngnncnnnn anancnnnna aaaaggcnnc nctgtgtnnn nngcgnnnnn nnnnaggnnt
     1081 ncnngccncc ccctgacnna gcatncncna aaaatcgann cntnnnangt cnnaangntg
     1141 gncnaaa
//
