LOCUS       sequence-61755-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GTGG
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(598..616)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(634..650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(634..650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(647..669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    658..674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(682..688,689..706,707..712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    727..748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(865..882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaattgggc cctctgatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat
       61 tcgcccttcg tcctagcgcg tctcggctca ccccagagca ggtcgtggca atcgcgagca
      121 ataacggcgg aaaacaggct ttggaaacgg tgcagaggct ccttccagtg ctgtgccaag
      181 cgcacggact caccccagag caggtcgtgg ccattgcctc gaatggaggg ggcaaacagg
      241 cgttggaaac cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc ctcaccccag
      301 agcaggtcgt ggcaatcgcg agcaataacg gcggaaaaca ggctttggaa acggtgcaga
      361 ggctccttcc agtgctgtgc caagcgcacg gattaacccc agagcaggtc gtggcaatcg
      421 cgagcaataa cggcggaaaa caggctttgg aaacggtgca gaggctcctt ccagtgctgt
      481 gccaagcgca cggactcaga gacgtgggca caggaaagaa gggcgaattc cagcacactg
      541 gcggccgtta ctagtggatc cgagctcggt accaagcttg atgcatagct tgagtattct
      601 atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ct
//
