LOCUS       sequence-61758-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-GTGT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(600..618)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(601..618)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(636..652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(649..671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    660..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(684..690,691..708,709..714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    729..750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaattgggcc ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat
       61 tcgcccttgc cgtcctagcg cgtctcggct caccccagag caggtcgtgg caatcgcgag
      121 caataacggc ggaaaacagg ctttggaaac ggtgcagagg ctccttccag tgctgtgcca
      181 agcgcacgga ttaaccccag agcaggtcgt ggccattgcc tcgaatggag ggggcaaaca
      241 ggcgttggaa accgtacaac gattgctgcc ggtgctgtgc caagcgcacg gcctcacccc
      301 agagcaggtc gtggcaatcg cgagcaataa cggcggaaaa caggctttgg aaacggtgca
      361 naggctcctt ccagtgctgt gccaagcgca cggattaacc ccagagcagg tcgtggccat
      421 tgcctcgaat ggagggggca aacaggcgtt ggaaaccgta caacgattgc tgccggtgct
      481 gtgccaagcg cacggactca gagacgtggg cacaggaaag aagggcgaat tccagcacac
      541 tggcggccgt tactagtgga tccgagctcg gtaccaagct tgatgcatag cttgagtatt
      601 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
//
