LOCUS       sequence-61759-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TATC
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(595..613)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(596..613)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(631..647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(631..647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(644..666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    655..671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(679..685,686..703,704..709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    724..745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(862..879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctctg atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttgcgcgtct cggctcaccc cagagcaggt cgtggccatt gcctcgaatg
      121 gagggggcaa acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc
      181 acggactcac cccagagcag gtcgtggcaa tcgcctccaa cattggcggg aaacaggcac
      241 tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggcctc accccagagc
      301 aggtcgtggc cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat
      361 tgctgccggt gctgtgccaa gcgcacggat taaccccaga gcaggtcgtg gcgatcgcaa
      421 gccacgacgg aggaaagcaa gccttggaaa cagtacagag gctgttgcct gtgctgtgcc
      481 aagcgcacgg actcagagac gtgggcacag gaaagaaggg cgaattccag cacactggcg
      541 gccgttacta gtggatccga gctcggtacc aagcttgatg catagcttga gtattctata
      601 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      901 gcgagcggta tcagctcact caaaggcggt aatacngtta tccacagaat caggggata
//
