LOCUS       sequence-61763-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TTAA
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(608..626)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(644..660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(644..660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(657..679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    668..684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(692..698,699..716,717..722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    737..758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(875..892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatnttgggc gattgggccc tctgatgcat gctcgagcgg ccgccagtgt gatggatatc
       61 tgcagaattc gcccttgccg tcctagcgcg tctcggctca ccccagagca ggtcgtggcc
      121 attgcctcga atggaggggg caaacaggcg ttggaaaccg tacaacgatt gctgccggtg
      181 ctgtgccaag cgcacggact caccccagag caggtcgtgg ccattgcctc gaatggaggg
      241 ggcaaacagg cgttggaaac cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc
      301 ctcaccccag agcaggtcgt ggcaatcgcc tccaacattg gcgggaaaca ggcactcgag
      361 actgtccagc gcctgcttcc cgtgctgtgc caagcgcacg gattaacccc agagcaggtc
      421 gtggcaatcg cctccaacat tggcgggaaa caggcactcg agactgtcca gcgcctgctt
      481 cccgtgctgt gccaagcgca cggactcaga gacgtgggca caggaaagaa gggcgaattc
      541 cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg atgcatagct
      601 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      901 tcgttcggct gcggcgagcg gtatcactca tcaagcgtan ngttcnaatc ggnacaaaat
      961 tacannanaa ttnnnnnnan aannnnnnna annaanntnn nnnannaaan ntgnnnnnnn
     1021 nngnnnnnan nnnntaaang nnnccttgnn tttttntttn tncnnanccc cccgnccnnt
     1081 tnnaaaaaan tnaannttna ntnnaaaant gnnaaanccc nanagaantt aaaaaattnn
     1141 nggnttttc
//
