LOCUS       sequence-61776-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TAGT
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(613..631)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(614..631)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(649..665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(649..665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(662..684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    673..689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(697..703,704..721,722..727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctctg atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttgatgccgt cctagcgcgt ctcggctcac cccagagcag gtcgtggcca
      121 ttgcctcgaa tggagggggc aaacaggcgt tggaaaccgt acaacgattg ctgccggtgc
      181 tgtgccaagc gcacggactc accccagagc aggtcgtggc aatcgcctcc aacattggcg
      241 ggaaacaggc actcgagact gtccagcgcc tgcttcccgt gctgtgccaa gcgcacggcc
      301 tcaccccaga gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag gctttggaaa
      361 cggtgcagag gctccttcca gtgctgtgcc aagcgcacgg attaacccca gagcaggtcg
      421 tggccattgc ctcgaatgga gggggcaaac aggcgttgga aaccgtacaa cgattgctgc
      481 cggtgctgtg ccaagcgcac ggactcagag acgtgggcac aggaaagata cttaagggcg
      541 aattccagca cactggcggc cgttactagt ggatccgagc tcggtaccaa gcttgatgca
      601 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt
      661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaaggcgtat acgttccaat
      961 caggnaccag aaa
//
