LOCUS       sequence-61782-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: III-TAGG
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(610..628)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(611..628)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(646..662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(646..662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(659..681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    670..686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(694..700,701..718,719..724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    739..760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(877..894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgattggg ccctctgatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgcccttg atgccgtcct agcgcgtctc ggctcacccc agagcaggtc gtggccattg
      121 cctcgaatgg agggggcaaa caggcgttgg aaaccgtaca acgattgctg ccggtgctgt
      181 gccaagcgca cggactcacc ccagagcagg tcgtggcaat cgcctccaac attggcggga
      241 aacaggcact cgagactgtc cagcgcctgc ttcccgtgct gtgccaagcg cacggcctca
      301 ccccagagca ggtcgtggca atcgcgagca ataacggcgg aaaacaggct ttggaaacgg
      361 tgcagaggct ccttccagtg ctgtgccaag cgcacggatt aaccccagag caggtcgtgg
      421 caatcgcgag caataacggc ggaaaacagg ctttggaaac ggtgcagagg ctccttccag
      481 tgctgtgcca agcgcacgga ctcagagacg tgggcacagg aaagatactt aagggcgaat
      541 tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccaagct tgatgcatag
      601 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      661 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      961 agaatcaggg gat
//
