LOCUS       sequence-61812-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pHD_v2
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..132)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        199..290
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc
       61 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt
      121 gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg
      181 ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagccata
      301 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      361 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
      421 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      481 cagatgagat ggtcagacta aactggctga cggaatttat gccacttccg accatcaagc
      541 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
      601 cgttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      661 tgttcctgcg ccggttgcac tcgattcctg tttgtaattg tccttttaac agcgatcgcg
      721 tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
      781 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
      841 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
      901 ttgacgaggg
//
