LOCUS       sequence-61815-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS337-USP44-FLAG
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(175..199)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(248..271)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(321..412)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(379..396)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     primer_bind     complement(732..754)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctcttcaat atatcttcca caggcaacat gggagcagct aaggcaagcc caaatggact
       61 cggtcgtgtt gcagtccaca cagtgccatt tctgagggtt gaggctggaa tggtcttgag
      121 caagctgcag ctgcccaaca tgtttgcacg tatccattgc tagcatggtg ggcgcgccaa
      181 cttttttgta caaacttgtt gatgggtacc agctgcagat ctcgagctcg gatcccatgg
      241 gcatatgctt gtcatcgtca tccttgtagt ccatggtggc tagcacgccc gatggtggga
      301 cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag
      361 taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg atatcaagcc
      421 acactggaca cgaaaaagtc gaaggcactc gcttcgatca atggacaaag aatcaatcgt
      481 tcgtaggcaa cagaatgtcg gaaagtttgc attggatgcg cggcgggtcc aacttgccgc
      541 aaaactgcgg cgagttcaac gtggtgtcca gcctgttgat gtgcaacaat acgataatga
      601 aaaattgata acgcttgcac gattgcaaac atgcacgctc ggttgaataa aagctcgcat
      661 cgtcgtcgta aaattagttg tatcaaagag cagctgcaat tagaatcact gctaaaaatg
      721 atagatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      781 cgccttgcag cacatccccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      841 tcgcccttcc aacagttgcg cagcctgaat ggcgaatggg cttttttgcc tggtttccgg
      901 caccagaagc cggtgcccga aagctggctg ga
//
