LOCUS       sequence-61817-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NN)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..77)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(99..248)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(273..293)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacagctcct cgcccttgct cactgggcca ggattctcct cgacgtcacc gcatgttagc
       61 agacttcctc tgccctctcc actgcctcta gagttaatca gcatgtccag gtcgaaatca
      121 tcaagggcgt cactgccgag catgtcgagg tcaaagtcat caagggcatc cgaaccaagc
      181 atgtcaaggt caaaatcatc gagggcgtca cttcccagca tatccagatc aaaatcgtcc
      241 aatgcgtcag cccgtccgga accgctggcc tccacctttc tcttcttctt ggggctagct
      301 gacgcgcgag tttggtcccc ttcatgcatg ggcgagggcg cgtccaaatc cctctccagc
      361 gagtctgcga acgcatgaag gctcgcctgg tcaggtgtct gagttgacgt aggtgaaggc
      421 ttcgcgcgtt tcataccgct cgcttggaga atcctgtccc accgctggga ggcgggaggc
      481 agtgtgcccg agcgggcctc gagttctgtg acaccgactc tacgaaagag ctgcagcagt
      541 ccgtgtctcg acataccaaa ttgagtcatg gcgtcatcga acgcttgtgc ggggtgggag
      601 tgacactgga agaatccgag cacgcggacc acttgcgcgt gatctgccac tcgatgtgat
      661 gtcctctcgg gaatccttct gttggtccgc ttaatcaatg caggcgcgtg cgggagcccc
      721 ttcttcaccg catccaaggc gggtcgtccg ccgaggcagg ccagcgctac aagatgatca
      781 ttagtgagtg cggccagcgc ggggtccggc ctcgaaagct gggccacgat tgactccagt
      841 gcgggtctgc ccccgttatt ggatgcaata gccactacct gctcaggcgt gagtcgagac
      901 cgtcgacctg cagactggct g
//
