LOCUS       sequence-61818-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS322-USP44-catDeadMyc
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(177..201)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          328..424
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(541..565)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(562..765)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(591..611)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgctcttca atatatcttc cacaggcaac atgggagcag ctaaggcaag cccaaatgga
       61 ctcggtcgtg ttgcagtcca cacagtgcca tttctgaggg ttgaggctgg aatggtcttg
      121 agcaagctgc agctgcccaa catgtttgca cgtatccatt gctagcatgg tgggcgcgcc
      181 aacttttttg tacaaacttg tgataattcg ggcctccatg gccataagca ggtcctcctc
      241 tgagatcagc ttctgcattg atgccatggt gggcccgcgg gtacaattcc gcagctttta
      301 gagcagaagt aacacttccg tacaggccta gaagtaaagg caacatccac tgaggagcag
      361 ttctttgatt tgcaccacca ccggatccgg gacctgaaat aaaagacaaa aagactaaac
      421 ttaccagtta actttctggt ttttcagttc ctctagtacc ggatcctcta gagtccggag
      481 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
      541 gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
      601 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
      661 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
      721 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
      781 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
      841 atgccaggcg ggccatttac cgtcattgac gtcaataagg ggcgtacttg gcatatgata
      901 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      961 gaaagtccct attggcgtta ctatgggaac a
//
