LOCUS       sequence-61820-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALETF_v2 (NI)
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..78)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(100..249)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(274..294)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaacagctcc tcgcccttgc tcactgggcc aggattctcc tcgacgtcac cgcatgttag
       61 cagacttcct ctgccctctc cactgcctct agagttaatc agcatgtcca ggtcgaaatc
      121 atcaagggcg tcactgccga gcatgtcgag gtcaaagtca tcaagggcat ccgaaccaag
      181 catgtcaagg tcaaaatcat cgagggcgtc acttcccagc atatccagat caaaatcgtc
      241 caatgcgtca gcccgtccgg aaccgctggc ctccaccttt ctcttcttct tggggctagc
      301 tgacgcgcga gtttggtccc cttcatgcat gggcgagggc gcgtccaaat ccctctccag
      361 cgagtctgcg aacgcatgaa ggctcgcctg gtcaggtgtc tgagttgacg taggtgaagg
      421 cttcgcgcgt ttcataccgc tcgcttggag aatcctgtcc caccgctggg aggcgggagg
      481 cagtgtgccc gagcgggcct cgagttctgt gacaccgact ctacgaaaga gctgcagcag
      541 tccgtgtctc gacataccaa attgagtcat ggcgtcatcg aacgcttgtg cggggtggga
      601 gtgacactgg aagaatccga gcacgcggac cacttgcgcg tgatctgcca ctcgatgtga
      661 tgtcctctcg ggaatccttc tgttggtccg cttaatcaat gcaggcgcgt gcgggagccc
      721 cttcttcacc gcatccaagg cgggtcgtcc gccgaggcag gccagcgcta caagatgatc
      781 attagtgagt gcggccagcg cggggtccgg cctcgaaagc tgggccacga ttgactccag
      841 tgcgggtctg cccccgatat tggatgcaat agccactacc tgctcaggcg tgagtcgaga
      901 ccgtcgacct gcagactggc tgtgtataa
//
