LOCUS       sequence-61824-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS331-USP44-catDead-HIS
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(181..201)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(283..300)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(316..333)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(380..398)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(381..398)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgcatgctct tcaatatatc ttccacaggc aacatgggag cagctaaggc aagcccaaat
       61 ggactcggtc gtgttgcagt ccacacagtg ccatttctga gggttgaggc tggaatggtc
      121 ttgagcaagc tgcagctgcc caacatgttt gcacgtatcc attgctagca tgggccggcc
      181 gccctggaag tacaggttct cgctgctgcc gggccggggc ttgccggcat cggcgccggc
      241 atccacggtg ccgctttgaa aatataaatt ttcccggcct gcatggtggt gatgatggtg
      301 ctgagaacct ctagcatggt gatggtgatg gtgtgatcct cgagccatgg atcctgcaaa
      361 aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc gacgtcccca
      421 ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt aggcacgcct
      481 accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc attgacgtca
      541 atggggatgt acttggcagc catcgcgggc catttaccgc cattgacgtc aatgggagta
      601 ctgccaatgt accctggcgt acttccaata gtaatgtact tgccaagtta ctattaatag
      661 atattgatgt actgccaagt gggccattta ccgtcattga cgtcaatagg gggcgtgaga
      721 acggatatga atgggcaatg agccatccca ttgacgtcaa tggtgggtgg tcctattgac
      781 gtcaatgggc attgagccag gcgggccatt taccgtaatt gacgtcaatg ggggaggcgc
      841 catatacgtc aataggaccg cccatatgac gtcaatagga aagaccatat atagagacca
      901 ttgacgtcaa tggggga
//
