LOCUS       sequence-61829-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pFS321-pDestMyc-USP44catDead
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    90..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(127..146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     181..200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    270..294
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcctgcagaa tcgactctag aggatccgaa aaaacctccc acacctcccc ctgaacctga
       61 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccgcg gccgcggtac
      241 ctcgagagat ctcggtcgac cgaattatca ccactttgta caagaaagtt gggtaattaa
      301 ttaatcagct aaggatttca ttagacgagg tatcagcgtc ttcattggga tgttggctcc
      361 ccaacaggag ctctggaggc aaaagtttag aatgtccatt ctcagtaact cgttgggtat
      421 aaaacaagat ataagcttga gccttgcata cttcgtccat agtgcacatg cttagtttgg
      481 aatcattgca gtgtacccag aaccctcctt cagaattata gcagtaggca gtgtagtgcc
      541 ctgagccaaa tcctttccca tggtgcatca ccaccgcgga caagtcatag ataaagcatt
      601 ctggtctgag ggatttcagg gtctccctgc agcaataggg ctccatgttt aagatttcct
      661 caaagccaac atgaacacca atcttctctc ggttattacg tcctgaccac ctgaatcgtt
      721 tgaggtgcag tctgagaacc tgaggtaggt ggcatatcat aagttgtttc tgggcttctg
      781 tgagtacaac tggtttggag gaaaaccttc tacgctttga gttacactgg tcacatacgt
      841 agatttttcc ttctaaagct tcagtttctg taaatttggc caacatttca gtaaccagac
      901 atggctggga agcaatatct tttccactgc attgatacct ttc
//
