LOCUS       sequence-61830-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-CTTC
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(605..623)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(606..623)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(641..657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(641..657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(654..676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    665..681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(689..695,696..713,714..719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    734..755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(872..889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttctttcct gtgcccacgt ctctgagccc gtgcgcttgg cacagcacag
      121 gcaacagcct ctgtactgtt tccaaggctt gctttcctcc gtcgtggctt gcgatcgcca
      181 cgacctgctc tggggttaat ccgtgcgctt ggcacagcac cggcagcaat cgttgtacgg
      241 tttccaacgc ctgtttgccc cctccattcg aggcaatggc cacgacctgc tctggggtga
      301 ggccgtgcgc ttggcacagc accggcagca atcgttgtac ggtttccaac gcctgtttgc
      361 cccctccatt cgaggcaatg gccacgacct gctctggggt gagtccgtgc gcttggcaca
      421 gcacaggcaa cagcctctgt actgtttcca aggcttgctt tcctccgtcg tggcttgcga
      481 tcgccacgac ctgctctggg gttaagcgag acgcgctagg acggcaaggg cgaattccag
      541 cacactggcg gccgttacta gtggatccga gctcggtacc aagcttgatg catagcttga
      601 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      661 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      721 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      781 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      841 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      961 caggggatan gcaggaaaaa aatgtnanca aaaagccacc aaa
//
