LOCUS       sequence-61831-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALEN_v2 (HD)
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             83..148
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             155..175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        join(644..649,650..667,668..674)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(794..813)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata ggggccacca tggactataa ggaccacgac ggagactaca aggatcatga
      121 tattgattac aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat
      181 ccacggagtc ccagcagccg tagatttgag aactttggga tattcacagc agcagcagga
      241 aaagatcaag cccaaagtga ggtcgacagt cgcgcagcat cacgaagcgc tggtgggtca
      301 tgggtttaca catgcccaca tcgtagcctt gtcgcagcac cctgcagccc ttggcacggt
      361 cgccgtcaag taccaggaca tgattgcggc gttgccggaa gccacacatg aggcgatcgt
      421 cggtgtgggg aaacagtgga gcggagcccg agcgcttgag gccctgttga cggtcgcggg
      481 agagctgaga gggcctcccc ttcagctgga cacgggccag ttgctgaaga tcgcgaagcg
      541 gggaggagtc acggcggtcg aggcggtgca cgcgtggcgc aatgcgctca cgggagcacc
      601 cctcaacctg acagagaccg cggccgcatt aggcacccca ggctttacac tttatgcttc
      661 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
      721 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      781 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      841 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      901 ctttattcac attcttgccc gcctgatgaa tg
//
