LOCUS       sequence-61835-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: II-CTGC
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(619..637)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(620..637)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(668..690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    679..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(703..709,710..727,728..733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    748..769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(886..903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttatataga tgccgtccta gcgcgtctcg cttaacccca gagcaggtcg
      121 tggcgatcgc aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc
      181 ctgtgctgtg ccaagcgcac ggactcaccc cagagcaggt cgtggccatt gcctcgaatg
      241 gagggggcaa acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc
      301 acggcctcac cccagagcag gtcgtggcaa tcgcgagcaa taacggcgga aaacaggctt
      361 tggaaacggt gcagaggctc cttccagtgc tgtgccaagc gcacggatta accccagagc
      421 aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc cttggaaaca gtacagaggc
      481 tgttgcctgt gctgtgccaa gcgcacgggc tcagagacgt gggcacagga aagatactta
      541 agggcgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
      601 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      721 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      781 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      841 acgcgcgggg agangcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      961 gttatccaca gaatcagggg nataacgcag gaaagaacat gtgagcaaaa ggccagcaag
     1021 cgactaagct cgt
//
